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Real Time PCR Detection System

Real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR/qrt-PCR) or kinetic polymerase chain reaction (KPCR), is a laboratory technique based on the PCR, which is used to amplify and simultaneously quantify a targeted DNA molecule. Sample quantitation by Real-Time PCR increases reliability of gene expression analyses, improve viral load assessment, and accelerates throughput in screening applications. Bio-Rad's real-time PCR detection systems are optical modules that allow target sequence via fluorescent detection chemistry.

Real Time PCR Detection System Guide:

Real-Time PCR Detection Systems

Features:	MiniOpticon™	My IQ™2	CFX-96™	CFX-384™
Excitation range	70–500 nm	470–545 nm	450–684 nm	450–650 nm
Light source	Light-emitting diode (LED) array (48 blue-green LEDs)	Tungsten-halogen lamp	6 LEDs in optics shuttle	5 LEDs in optics shuttle
Optical detection	2 photodiodes	12-bit CCD camera	6-photodiodes	5 photodiodes
Detection range	523–700 nm	515–585 nm	515–730 nm	515–690 nm
Multiplex capability	Up to 2 targets	Up to 2 targets	Up to 5 targets	Up to 4 targets
Gradient range	35–99°C	40–100°C	30–100°C	30–100°C
Maximum gradient span	16°C	25°C	24°C	24°C
Maximum ramp rate	2.5°C/sec	3.3°C/sec	5°C/sec	2.5°C/sec
Dimensions (W x D x H)	18 x 32 x 33 cm	29 x 58 x 39 cm	33 x 46 x 36 cm	33 x 46 x 36 cm
Weight	6.8 kg	17.6 kg	21.4 kg	21.4 kg
Base thermal cycler	MJ Mini	iCycler	C1000	C1000